Stellaris
Manufacturer: Leica Microsystems
Model: Stellaris 5 (Point Scanning Confocal Microscope)
Applications:
• fixed samples (fluorescently labelled)
• Microscope live-cell imaging
body:
• Leica DMi8 platform
• Inverted
• LED light source - filter cubes:
o DAPI
o GFP
o RHOD
• Brightfield light source
• Transmitted light detector
• Motorized xyz scanning stage
o Universal flexible specimen frame
o Live-cell imaging chambers (temperature, CO2 and humidity control)
Plate
3 cm dish
Lasers:
• Tunable White Light Laser Stellaris 5 (485 – 685 nm)
• 405 nm laser
Detectors:
• 5 Power Hybrid HyD S detectors
o Analog, Reflection and Photon Counting detection mode
o Tau Sense capabilities: TauGating, TauContrast, TauSeparation
Objectives:
• 10X HC PL FLUOTAR (NA 0.3; DRY; WD 11 mm)
• 20X HC PL APO (NA 0.75; IMM Water, Gly, Oil; WD 0.66 mm; CS2)
• 40X HC PL APO (NA 1.1; WATER; WD 0.65 mm; CS2)
• 63X HC PL APO (NA 1.4; OIL; WD 0.14 mm; CS2)
Scanner, Speed, Resolution:
• 8 kHz Tandem Scanner – switchable between:
o Non-resonant mode:
confocal point scanner for highest axial resolution (adjustable speed 1-2600 Hz)
up to 10 fps 512 x 512 px
up to 5200 lines/second
max resolution 8192 x 8192 px
zoom 0.75X – 48X
scanfield: 22 mm diagonal
o Resonant mode:
adjustable speed up to 8 000 Hz
up to 28 fps at 512 x 512 px
max resolution 2496 x 2496 px with up to 3 channels simultaneously
zoom 1.25X – 48X
scanfield: 13 mm diagonal
Okolab incubation chamber:
• Temperature control
• Humidity control
• CO2 control
*The system is equipped with active liquid cooling and mounted on an air table.
Software:
• LAS X
o Image Compass: routine multichannel/Z-stack acquisition
o Navigator basic package: tile scan, merge
o LAS X 3D Visualization
o TauSense capabilities: TauGating, TauContrast, TauScan
o Dynamic Signal Enhancement
o LIGHTNING basic detection package
o LAS X Live Data Mode
o LAS X Assay Editor
o LAS X Dye Finder