SP8

Manufacturer:Leica TCS SP8 X point scanning confocal microscope

Objective:
10x NA 0.30 Air
20x NA 0.75 Air
20x NA 0.75 corr. multi immersion glycerol and Oil
40x NA 1.1 corr. water immersion
63x NA 1.3 corr. Glycerol immersion

Microscope Body: invers DMi8 CS

Laser:
405 nm, direct modulated
White light laser 470 – 670 nm, AOTF modulated

Scanner:
8 kHz tandem scanner
Standard mode: up to 7 images/s (512x512 px)
Resonant mode: up to 28 images/s (512x512 px)
up to 290 images/s (512x16 px)

Scan optics: HVIS with scan – rotation

Spectral detection:
AOBS and AOBM filter free spectral detection of the confocal signal

Detector:
1x PMT
2x HyD SP GaAsP

Software:
LAS X SP8 Control Software
LAS X Dye Finder
LAS X 3D Visualisation
Lightning deconvolution module
LAS X Assay Editor

Whitfield Fluorescence
SOLA - SMII, LED light source
Filter cubes: DAPI, FITC, Rhodamine

Interference contrast